MED28 antibody (AA 20-100)

Catalog No. ABIN7229628

The Rabbit Polyclonal anti-MED28 antibody (ABIN7229628) specifically detects MED28 in ELISA and WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MED28 antibody (AA 20-100) (ABIN7229628)

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED28 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-MED28 Antibody

Binding Specificity: AA 20-100

Purpose: Rabbit Anti-MED28 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of MED28 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human MED28 protein at AA range: 20-100

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MED28

Target: MED28 (Mediator Complex Subunit 28 (MED28))

Alternative Name: MED28

Background: Mediator of RNA polymerase II transcription subunit 28, Endothelial-derived protein 1, Mediator complex subunit 28, Merlin and Grb2-interacting cytoskeletal protein, Magicin, Tumor angiogenesis marker EG-1MED28 (Mediator Complex Subunit 28) is a Protein Coding gene. Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.

Molecular Weight: 19kD

Gene ID: 80306

UniProt: Q9H204

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation