MRPL10 antibody (AA 180-260)

Catalog No. ABIN7215828

The Rabbit Polyclonal anti-MRPL10 antibody is suitable to detect MRPL10 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for MRPL10 antibody (AA 180-260) (ABIN7215828)

Target: MRPL10 (Mitochondrial Ribosomal Protein L10 (MRPL10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MRPL10 Antibody

Binding Specificity: AA 180-260

Purpose: Rabbit Anti-MRP-L10 Polyclonal Antibody

Specificity: MRP-L10 Polyclonal Antibody detects endogenous levels of MRP-L10 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human MRP-L10 at AA range: 180-260

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MRPL10

Target: MRPL10 (Mitochondrial Ribosomal Protein L10 (MRPL10))

Alternative Name: MRP-L10

Background: MRPL10, MRPL8, RPML8, 39S ribosomal protein L10, mitochondrial, L10mt, MRP-L10, 39S ribosomal protein L8, mitochondrial, L8mt, MRP-L839S ribosomal protein L10 mitochondrial (Mammalian mitochondrial ribosomal protein L 10) is encoded by nuclear gene MRPL10 and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode two different isoforms. A pseudogene corresponding to this gene is found on chromosome 5q.

Molecular Weight: 29kD

Gene ID: 124995

UniProt: Q7Z7H8