MRPS33 antibody (AA 30-110)

Catalog No. ABIN7223082

This Rabbit Polyclonal antibody specifically detects MRPS33 in IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MRPS33 antibody (AA 30-110) (ABIN7223082)

Target: MRPS33 (Mitochondrial Ribosomal Protein S33 (MRPS33))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPS33 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MRPS33 Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-MRP-S33 Polyclonal Antibody

Specificity: MRP-S33 Polyclonal Antibody detects endogenous levels of MRP-S33 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human MRP-S33 at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MRPS33

Target: MRPS33 (Mitochondrial Ribosomal Protein S33 (MRPS33))

Alternative Name: MRP-S33

Background: MRPS33, CGI-139, PTD003, 28S ribosomal protein S33, mitochondrial, MRP-S33, S33mtMammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. MRPS33 (mitochondrial ribosomal protein S33) encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene, all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q.

Gene ID: 51650

UniProt: Q9Y291