MAPK15 antibody (pThr175)

Catalog No. ABIN7220514

This anti-MAPK15 antibody is a Rabbit Polyclonal antibody detecting MAPK15 in WB, ELISA and IHC (p). Suitable for Human and Mouse.

Quick Overview for MAPK15 antibody (pThr175) (ABIN7220514)

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAPK15 Antibody

Binding Specificity: pThr175

Purpose: ERK 8 (phospho Thr175/Y177) Polyclonal Antibody

Specificity: Phospho-ERK 8 (T175/Y177) Polyclonal Antibody detects endogenous levels of ERK 8 protein only when phosphorylated at T175/Y177.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human ERK 8 Phospho-Thr175/Y177

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MAPK15

Target: MAPK15 (Mitogen-Activated Protein Kinase 15 (MAPK15))

Alternative Name: ERK 8

Background: Rabbit Anti-ERK 8 (phospho Thr175/Y177) Polyclonal Antibody,MAPK15, ERK7, ERK8, Mitogen-activated protein kinase 15, MAP kinase 15, MAPK 15, Extracellular signal-regulated kinase 7, ERK-7, Extracellular signal-regulated kinase 8, ERK-8,The ERKs are a subfamily of the MAPKs that have been implicated in cell growth and differentiation. Extracellular signal-regulated kinase 8 (Erk8) is a large MAP kinase whose activity is controlled by serum and the c-Src non-receptor tyrosine kinase. ERK8 down-regulates transactivation of the glucocorticoid receptor through Hic-5 and can negatively regulate transcriptional co-activation of androgen receptor and GRalpha by Hic-5 in a kinase-independent manner, suggesting a broader role for ERK8 in the regulation of nuclear receptors beyond estrogen receptor alpha. Erk8 is a novel effector of RET/PTC3 and, therefore, RET biological functions.,Mitogen-activated protein kinase 15

Molecular Weight: observerd band 60kDa

Gene ID: 225689

UniProt: Q8TD08