MAPK8IP1 antibody (pThr103)

Catalog No. ABIN7225994

The Rabbit Polyclonal anti-MAPK8IP1 antibody is suitable to detect MAPK8IP1 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for MAPK8IP1 antibody (pThr103) (ABIN7225994)

Target: MAPK8IP1 (Mitogen-Activated Protein Kinase 8 Interacting Protein 1 (MAPK8IP1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK8IP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-MAPK8IP1 Antibody

Binding Specificity: pThr103

Purpose: Rabbit Anti-JIP-1 (phospho Thr103) Polyclonal Antibody

Specificity: Phospho-JIP-1 (T103) Polyclonal Antibody detects endogenous levels of JIP-1 protein only when phosphorylated at T103.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human JIP-1 Phospho-Thr103

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAPK8IP1

Target: MAPK8IP1 (Mitogen-Activated Protein Kinase 8 Interacting Protein 1 (MAPK8IP1))

Alternative Name: JIP-1

Background: MAPK8IP1, IB1, JIP1, PRKM8IP, C-Jun-amino-terminal kinase-interacting protein 1, JIP-1, JNK-interacting protein 1, Islet-brain 1, IB-1, JNK MAP kinase scaffold protein 1, Mitogen-activated protein kinase 8-interacting protein 1MAPK8IP1 (mitogen-activated protein kinase 8 interacting protein 1) encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. MAPK8IP1 is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes.

Molecular Weight: 113kD

Gene ID: 9479

UniProt: Q9UQF2