MAP4K2 antibody (AA 400-480)

Catalog No. ABIN7229498

The Rabbit Polyclonal anti-MAP4K2 antibody is suitable to detect MAP4K2 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for MAP4K2 antibody (AA 400-480) (ABIN7229498)

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MAP4K2 Antibody

Binding Specificity: AA 400-480

Purpose: Rabbit Anti-M4K2 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of M4K2 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human M4K2 protein at AA range: 400-480

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for MAP4K2

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Alternative Name: M4K2

Background: Mitogen-activated protein kinase kinase kinase kinase 2, B lymphocyte serine/threonine-protein kinase, Germinal center kinase, GC kinase, MAPK/ERK kinase kinase kinase 2, MEK kinase kinase 2, MEKKK 2, Rab8-interacting proteinThe protein encoded by MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2) is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1. Alternative splicing results in multiple transcript variants. MAP4K2 is a Protein Coding gene. Diseases associated with MAP4K2 include Cerebral Cavernous Malformations-2. Among its related pathways are TGF-Beta Pathway and MAPK signaling pathway.

Molecular Weight: 90kD

Gene ID: 5871

UniProt: Q12851