MAPKAP Kinase 2 antibody (pThr222)

Catalog No. ABIN7225910

This Rabbit Polyclonal antibody specifically detects MAPKAP Kinase 2 in WB, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for MAPKAP Kinase 2 antibody (pThr222) (ABIN7225910)

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPKAP Kinase 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MAPKAP Kinase 2 Antibody

Binding Specificity: pThr222

Purpose: MAPKAPK-2 (phospho Thr222) Polyclonal Antibody

Specificity: Phospho-MAPKAPK-2 (T222) Polyclonal Antibody detects endogenous levels of MAPKAPK-2 protein only when phosphorylated at T222.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human MAPKAPK-2 Phospho-Thr222

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Alternative Name: MAPKAPK-2

Background: Rabbit Anti-MAPKAPK-2 (phospho Thr222) Polyclonal Antibody,MAPKAPK2, MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAP-K2, MAPKAPK-2, MK-2, MK2,MAPKAPK2 (mitogen-activated protein kinase-activated protein kinase 2) encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for MAPKAPK2.,MAP kinase-activated protein kinase 2

Molecular Weight: observerd band 45kDa

Gene ID: 9261

UniProt: P49137

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades