NSF antibody (AA 110-190)

Catalog No. ABIN7222820

The Rabbit Polyclonal anti-NSF antibody is suitable to detect NSF in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for NSF antibody (AA 110-190) (ABIN7222820)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NSF Antibody

Binding Specificity: AA 110-190

Purpose: Rabbit Anti-NSF Polyclonal Antibody

Specificity: NSF Polyclonal Antibody detects endogenous levels of NSF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human NSF at AA range: 110-190

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: NSF

Background: NSF, Vesicle-fusing ATPase, N-ethylmaleimide-sensitive fusion protein, NEM-sensitive fusion protein, Vesicular-fusion protein NSFThe N-ethylmaleimide-sensitive fusion protein (NSF) is a 80 kDa cytosol protein that is expressed in all cell types. NSF functions as an ATPase and it is a part of the 20S membrane fusion apparatus with the vesicular SNAP receptor (v-SNARE) synaptobrevin, and the target SNAREs (t-SNARE) syntaxin and SNAP-25. With the hydrolysis of ATP by NSF, the released energy may set-up or drive membrane fusion processes. NSF is required for general membrane transport and trafficking.

Molecular Weight: 75kD

Gene ID: 4905

UniProt: P46459