NCK1 antibody

Catalog No. ABIN7229918

This Rabbit Polyclonal antibody specifically detects NCK1 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for NCK1 antibody (ABIN7229918)

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NCK1 Antibody

Purpose: Rabbit Anti-NCK1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of NCK1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human NCK1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NCK1

Target: NCK1 (NCK Adaptor Protein 1 (NCK1))

Alternative Name: NCK1

Background: Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alphaNCK1 (NCK Adaptor Protein 1) is a Protein Coding gene. Diseases associated with NCK1 include Wiskott-Aldrich Syndrome and Multiple Endocrine Neoplasia Iib. Among its related pathways are Netrin-1 signaling and Arf6 signaling events. The protein encoded by NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.

Molecular Weight: 41kD

Gene ID: 4690

UniProt: P16333

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals