NDC80 antibody (AA 320-400)

Catalog No. ABIN7218976

The Rabbit Polyclonal anti-NDC80 antibody is suitable to detect NDC80 in samples from Human and Mouse. It has been validated for WB, ELISA and IF.

Quick Overview for NDC80 antibody (AA 320-400) (ABIN7218976)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-NDC80 Antibody

Binding Specificity: AA 320-400

Purpose: Rabbit Anti-Hec1 Polyclonal Antibody

Specificity: Hec1 Polyclonal Antibody detects endogenous levels of Hec1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Hec1 at AA range: 320-400

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: Hec1

Background: NDC80, HEC, HEC1, KNTC2, Kinetochore protein NDC80 homolog, Highly expressed in cancer protein, Kinetochore protein Hec1, HsHec1, Kinetochore-associated protein 2, Retinoblastoma-associated protein HECNDC80 encodes a component of the NDC80 kinetochore complex. The NDC80, kinetochore complex component consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation.

Molecular Weight: 73kD

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location