Nerve Growth Factor antibody (Internal Region)

Catalog No. ABIN7216080

The Rabbit Polyclonal anti-Nerve Growth Factor antibody is suitable to detect Nerve Growth Factor in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for Nerve Growth Factor antibody (Internal Region) (ABIN7216080)

Target: Nerve Growth Factor (NGF)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Nerve Growth Factor Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-NGF Polyclonal Antibody

Specificity: NGF Polyclonal Antibody detects endogenous levels of NGF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human NGF

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:100-300,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: NGF, NGFB, Beta-nerve growth factor, Beta-NGFNGF is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. Nerve growth factorp has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in NGF have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of NGF's expression is associated with allergic rhinitis.

Molecular Weight: 27kD

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size