NCALD antibody (AA 370-450)

Catalog No. ABIN7225346

This Rabbit Polyclonal antibody specifically detects NCALD in ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NCALD antibody (AA 370-450) (ABIN7225346)

Target: NCALD (Neurocalcin delta (NCALD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCALD antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-NCALD Antibody

Binding Specificity: AA 370-450

Purpose: Rabbit Anti-Neurocalcin δ Polyclonal Antibody

Specificity: Neurocalcin δ Polyclonal Antibody detects endogenous levels of Neurocalcin δ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Neurocalcin delta at AA range: 370-450

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NCALD

Target: NCALD (Neurocalcin delta (NCALD))

Alternative Name: Neurocalcin delta

Background: NCALD, Neurocalcin-deltaNCALD (neurocalcin delta) encodes a member of the neuronal calcium sensor (NCS) family of calcium-binding proteins. The protein contains an N-terminal myristoylation signal and four EF-hand calcium binding loops. The protein is cytosolic at resting calcium levels, however, elevated intracellular calcium levels induce a conformational change that exposes the myristoyl group, resulting in protein association with membranes and partial co-localization with the perinuclear trans-golgi network. The protein is thought to be a regulator of G protein-coupled receptor signal transduction. Several alternatively spliced variants of NCALD have been determined, all of which encode the same protein, additional variants may exist but their biological validity has not been determined.

Gene ID: 83988

UniProt: P61601