NEFL antibody (C-Term)

Catalog No. ABIN7217742

The Rabbit Polyclonal anti-NEFL antibody (ABIN7217742) specifically detects NEFL in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NEFL antibody (C-Term) (ABIN7217742)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEFL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NEFL Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-NF-L Polyclonal Antibody

Specificity: NF-L Polyclonal Antibody detects endogenous levels of NF-L protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human NF-L

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Alternative Name: NF-L

Background: NEFL, NF68, NFL, Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L proteinNeurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Molecular Weight: 61kD

Gene ID: 4747

UniProt: P07196