NSG2 antibody (AA 10-90)

Catalog No. ABIN7223076

This Rabbit Polyclonal antibody specifically detects NSG2 in IHC, ELISA, WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for NSG2 antibody (AA 10-90) (ABIN7223076)

Target: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSG2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NSG2 Antibody

Binding Specificity: AA 10-90

Purpose: Rabbit Anti-HMP19 Polyclonal Antibody

Specificity: HMP19 Polyclonal Antibody detects endogenous levels of HMP19 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human HMP19 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NSG2

Target: NSG2 (HMP19) (Neuron-Specific Protein Family Member 2 (HMP19))

Alternative Name: HMP19

Background: NSG2, Neuron-specific protein family member 2, Protein p19, Hmp19The chondroitin sulfate proteoglycan NG2 is a type I membrane protein expressed by subpopulations of glia including oligodendroglial precursor cells and a variety of tumor cells. Normal precursor cells and malignant tumor cells migrate and proliferate, but there is evidence that cells may not be able to engage in both activities at the same time. However, NG2 is involved in promoting both proliferation and motility. The extracellular domain of NG2 sequesters growth factors and binds to both growth factor receptors and extracellular matrix ligands such as fibronectin, collagens and laminin. The cytoplasmic domain is involved in activating Rac, Cdc42 and p130 Cas. PKCα phosphorylates NG2 at Thr2256, triggering the redistribution of NG2 from apical microprocesses to lamellipodia accompanied by enhanced cell motility. ERK phosphorylates NG2 at Thr2314, stimulating cell proliferation.

Molecular Weight: 19kD

Gene ID: 51617

UniProt: Q9Y328