NCF1 antibody (pSer359)

Catalog No. ABIN7224364

This anti-NCF1 antibody is a Rabbit Polyclonal antibody detecting NCF1 in WB, ELISA, IF and IHC (p). Suitable for Human.

Quick Overview for NCF1 antibody (pSer359) (ABIN7224364)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NCF1 Antibody

Binding Specificity: pSer359

Purpose: p47-phox (phospho Ser359) Polyclonal Antibody

Specificity: Phospho-p47-phox (S359) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S359.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human p47-phox Phospho-Ser359

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: p47-phox

Background: Rabbit Anti-p47-phox (phospho Ser359) Polyclonal Antibody,NCF1, NOXO2, SH3PXD1A, Neutrophil cytosol factor 1, NCF-1, 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-47K, Neutrophil NADPH oxidase factor 1, Nox organizer 2, Nox-organizing protein 2, SH3,The protein encoded by NCF1 (neutrophil cytosolic factor 1) is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in NCF1 have been associated with chronic granulomatous disease.,Neutrophil cytosol factor 1

Molecular Weight: observerd band 45kDa

Gene ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signaling