NEK1 antibody (AA 280-360)

Catalog No. ABIN7229954

This Rabbit Polyclonal antibody specifically detects NEK1 in ELISA and WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for NEK1 antibody (AA 280-360) (ABIN7229954)

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-NEK1 Antibody

Binding Specificity: AA 280-360

Purpose: Rabbit Anti-NEK1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of NEK1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human NEK1 protein at AA range: 280-360

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NEK1

Target: NEK1 (NIMA-Related Kinase 1 (NEK1))

Alternative Name: NEK1

Background: Serine/threonine-protein kinase Nek1, Never in mitosis A-related kinase 1, NimA-related protein kinase 1, Renal carcinoma antigen NY-REN-55The protein encoded by NEK1 (NIMA Related Kinase 1) is a serine/threonine kinase involved in cell cycle regulation. The encoded protein is found in a centrosomal complex with FEZ1, a neuronal protein that plays a role in axonal development. Defects in NEK1 are a cause of polycystic kidney disease (PKD). Several transcript variants encoding different isoforms have been found for NEK1.

Molecular Weight: 138kD

Gene ID: 4750

UniProt: Q96PY6