NOTCH2 antibody (Cleaved-Ala1734)

Catalog No. ABIN7222796

The Rabbit Polyclonal anti-NOTCH2 antibody has been validated for WB and ELISA. It is suitable to detect NOTCH2 in samples from Human, Mouse and Rat.

Quick Overview for NOTCH2 antibody (Cleaved-Ala1734) (ABIN7222796)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NOTCH2 Antibody

Binding Specificity: AA 1690-1770, Cleaved-Ala1734

Purpose: Cleaved-Notch 2 (A1734) Polyclonal Antibody

Specificity: Cleaved-Notch 2 (A1734) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 2 protein resulting from cleavage adjacent to A1734.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Notch 2 at AA range: 1690-1770

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: Notch 2

Background: Rabbit Anti-Cleaved-Notch 2 (A1734) Polyclonal Antibody,NOTCH2, Neurogenic locus notch homolog protein 2, Notch 2, hN2,NOTCH2 encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. The notch 2 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. The notch 2 functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.,Neurogenic locus notch homolog protein 2

Molecular Weight: observerd band 79kDa

Gene ID: 4853

UniProt: Q04721

Pathways: Notch Signaling, Stem Cell Maintenance