NOTCH4 antibody (Cleaved-Val1432)

Catalog No. ABIN7222800

This Rabbit Polyclonal antibody specifically detects NOTCH4 in WB and ELISA. It exhibits reactivity toward Human and Monkey.

Quick Overview for NOTCH4 antibody (Cleaved-Val1432) (ABIN7222800)

Target: NOTCH4 (Notch 4 (NOTCH4))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NOTCH4 Antibody

Binding Specificity: AA 1370-1450, Cleaved-Val1432

Purpose: Cleaved-Notch 4 (V1432) Polyclonal Antibody

Specificity: Cleaved-Notch 4 (V1432) Polyclonal Antibody detects endogenous levels of fragment of activated Notch 4 protein resulting from cleavage adjacent to V1432.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Notch 4 at AA range: 1370-1450

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: Notch 4

Background: Rabbit Anti-Cleaved-Notch 4 (V1432) Polyclonal Antibody,NOTCH4, INT3, Neurogenic locus notch homolog protein 4, Notch 4, hNotch4,NOTCH4 encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor may play a role in vascular, renal and hepatic development. Mutations in NOTCH4 may be associated with schizophrenia. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.,Neurogenic locus notch homolog protein 4

Molecular Weight: observerd band 59kDa

Gene ID: 4855

UniProt: Q99466

Pathways: Notch Signaling