NRF2 antibody (acLys599)

Catalog No. ABIN7212658

This anti-NRF2 antibody is a Rabbit Polyclonal antibody detecting NRF2 in WB, ELISA and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for NRF2 antibody (acLys599) (ABIN7212658)

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-NRF2 Antibody

Binding Specificity: acLys599

Purpose: Nrf2 (Acetyl Lys599) Polyclonal Antibody

Specificity: Acetyl-Nrf2 (K599) Polyclonal Antibody detects endogenous levels of Nrf2 protein only when non-acetylation at K599.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Nrf2 around the acetylation site of K599

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: Nrf2

Background: Rabbit Anti-Nrf2 (Acetyl Lys599) Polyclonal Antibody,NFE2L2, NRF2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2,NFE2L2 encodes a transcription factor (nuclear factor, erythroid 2 like 2) which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters, many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for NFE2L2.,Nuclear factor erythroid 2-related factor 2

Molecular Weight: observerd band 75-100kDa

Gene ID: 4780

UniProt: Q16236

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling