NUMA1 antibody (AA 310-390)

Catalog No. ABIN7222852

The Rabbit Polyclonal anti-NUMA1 antibody (ABIN7222852) specifically detects NUMA1 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for NUMA1 antibody (AA 310-390) (ABIN7222852)

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NUMA1 Antibody

Binding Specificity: AA 310-390

Purpose: Rabbit Anti-NuMA Polyclonal Antibody

Specificity: NuMA Polyclonal Antibody detects endogenous levels of NuMA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human NuMA at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NUMA1

Target: NUMA1 (Nuclear Mitotic Apparatus Protein 1 (NUMA1))

Alternative Name: NuMA

Background: NUMA1, NUMA, Nuclear mitotic apparatus protein 1, NuMA protein, SP-H antigenNUMA1 encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of NUMA1 with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants.

Molecular Weight: 240kD

Gene ID: 4926

UniProt: Q14980

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, M Phase