NARF antibody (AA 230-310)

Catalog No. ABIN7221022

This Rabbit Polyclonal antibody specifically detects NARF in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NARF antibody (AA 230-310) (ABIN7221022)

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NARF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NARF Antibody

Binding Specificity: AA 230-310

Purpose: Rabbit Anti-NARF Polyclonal Antibody

Specificity: NARF Polyclonal Antibody detects endogenous levels of NARF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human NARF at AA range: 230-310

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for NARF

Target: NARF (Nuclear Prelamin A Recognition Factor (NARF))

Alternative Name: NARF

Background: NARF, Nuclear prelamin A recognition factor, Iron-only hydrogenase-like protein 2, IOP2Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The nuclear prelamin A recognition factor encoded by NARF binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing.

Molecular Weight: 51kD

Gene ID: 26502

UniProt: Q9UHQ1