OGT antibody

Catalog No. ABIN7230192

This Rabbit Polyclonal antibody specifically detects OGT in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for OGT antibody (ABIN7230192)

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OGT antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-OGT Antibody

Purpose: OGT Polyclonal Antibody

Specificity: This antibody detects endogenous levels of OGT.

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen

Immunogen: Synthesized peptide derived from part region of human OGT protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: OGT

Background: Rabbit Anti-OGT Polyclonal Antibody,UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, O-GlcNAc transferase subunit p110, O-linked N-acetylglucosamine transferase 110 kDa subunit, OGT,OGT encodes a glycosyltransferase that catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains multiple tetratricopeptide repeats that are required for optimal recognition of substrates. Alternatively spliced transcript variants encoding distinct isoforms have been found forOGT.,OGT

Molecular Weight: observerd band 115kDa

Gene ID: 8473

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process