OPN4 antibody (AA 400-480)

Catalog No. ABIN7215736

The Rabbit Polyclonal anti-OPN4 antibody (ABIN7215736) specifically detects OPN4 in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for OPN4 antibody (AA 400-480) (ABIN7215736)

Target: OPN4 (Opsin 4 (OPN4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OPN4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-OPN4 Antibody

Binding Specificity: AA 400-480

Purpose: Rabbit Anti-Melanopsin Polyclonal Antibody

Specificity: Melanopsin Polyclonal Antibody detects endogenous levels of Melanopsin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Melanopsin at AA range: 400-480

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for OPN4

Target: OPN4 (Opsin 4 (OPN4))

Alternative Name: Melanopsin

Background: OPN4, MOP, Melanopsin, Opsin-4Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. OPN4 encodes a photoreceptive opsin protein melanopsin that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. Melanopsin functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 55kD

Gene ID: 94233

UniProt: Q9UHM6