PAK2 antibody (pSer197)

Catalog No. ABIN7222918

This Rabbit Polyclonal antibody specifically detects PAK2 in ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PAK2 antibody (pSer197) (ABIN7222918)

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAK2 Antibody

Binding Specificity: pSer197

Purpose: PAKγ (phospho Ser197) Polyclonal Antibody

Specificity: Phospho-PAKγ (S197) Polyclonal Antibody detects endogenous levels of PAKγ protein only when phosphorylated at S197.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PAKgamma Phospho-Ser197

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PAK2

Target: PAK2 (P21-Activated Kinase 2 (PAK2))

Alternative Name: PAKgamma

Background: Rabbit Anti-PAKγ (phospho Ser197) Polyclonal Antibody,PAK2, Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58,The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by PAK2 is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.,serine threonine-protein kinase PAK 2

Gene ID: 5062

UniProt: Q13177

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha