PAK4 antibody

Catalog No. ABIN7218956

This Rabbit Polyclonal antibody specifically detects PAK4 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PAK4 antibody (ABIN7218956)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PAK4 Antibody

Purpose: PAK4/5/6 Polyclonal Antibody

Specificity: PAK4/5/6 Polyclonal Antibody detects endogenous levels of PAK4/5/6 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PAK4 around the non-phosphorylation site of S474

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4/5/6

Background: Rabbit Anti-PAK4/5/6 Polyclonal Antibody,PAK4, KIAA1142, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4, PAK-4,PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK5 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.,serine threonine-protein kinase PAK 4

Molecular Weight: observerd band 80kDa

Gene ID: 10298

UniProt: O96013

Pathways: RTK Signaling