PAKalpha/beta/gamma antibody (pThr402, pThr421, pThr423)

Catalog No. ABIN7222902

This anti-PAKalpha/beta/gamma antibody is a Rabbit Polyclonal antibody detecting PAKalpha/beta/gamma in ELISA, WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for PAKalpha/beta/gamma antibody (pThr402, pThr421, pThr423) (ABIN7222902)

Target: PAKalpha/beta/gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAKalpha/beta/gamma antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAKalpha/beta/gamma Antibody

Binding Specificity: pThr402, pThr421, pThr423

Purpose: PAKα/β/γ (phospho Thr423/402/421) Polyclonal Antibody

Specificity: Phospho-PAKα/β/γ (T423/402/421) Polyclonal Antibody detects endogenous levels of PAKα/β/γ protein only when phosphorylated at T423/402/421.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PAKalpha/beta/gamma Phospho-Thr423/402/421

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PAKalpha/beta/gamma

Target: PAKalpha/beta/gamma

Background: Rabbit Anti-PAKα/β/γ (phospho Thr423/402/421) Polyclonal Antibody,PAK1, Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK2, Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58, PAK3, OPHN3, Serine/threonine-p,PAK1 encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.,serine threonine-protein kinase PAK 1

Molecular Weight: observerd band 60kDa

Gene ID: 5058, 5062, 5063