PAKalpha/beta/gamma antibody

Catalog No. ABIN7222904

This Rabbit Polyclonal antibody specifically detects PAKalpha/beta/gamma in ELISA, IHC, WB and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PAKalpha/beta/gamma antibody (ABIN7222904)

Target: PAKalpha/beta/gamma

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAKalpha/beta/gamma antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PAKalpha/beta/gamma Antibody

Purpose: Rabbit Anti-PAKα/β/γ Polyclonal Antibody

Specificity: PAKα/β/γ Polyclonal Antibody detects endogenous levels of PAKα/β/γ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PAKalpha/beta/gamma around the non-phosphorylation site of T423/402/421

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PAKalpha/beta/gamma

Target: PAKalpha/beta/gamma

Background: PAK1, Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK2, Serine/threonine-protein kinase PAK 2, Gamma-PAK, PAK65, S6/H4 kinase, p21-activated kinase 2, PAK-2, p58, PAK3, OPHN3, Serine/threonine-pPAK1 encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 62kD

Gene ID: 5058, 5062, 5063

UniProt: Q13153, Q13177, O75914