Parkin antibody

Catalog No. ABIN7216454

The Rabbit Polyclonal anti-Parkin antibody (ABIN7216454) specifically detects Parkin in WB, ELISA and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Parkin antibody (ABIN7216454)

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Parkin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Parkin Antibody

Purpose: Parkin Polyclonal Antibody

Specificity: Parkin Polyclonal Antibody detects endogenous levels of Parkin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Parkin around the non-phosphorylation site of S131

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Parkin

Target: Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))

Alternative Name: Parkin

Background: Rabbit Anti-Parkin Polyclonal Antibody,PARK2, PRKN, E3 ubiquitin-protein ligase parkin, Parkinson juvenile disease protein 2, Parkinson disease protein 2,The precise function of PARK2 is unknown, however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in PARK2 are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of PARK2 produces multiple transcript variants encoding distinct isoforms. Additional splice variants of PARK2 have been described but currently lack transcript support.,E3 ubiquitin-protein ligase parkin

Molecular Weight: observerd band 55kDa

Gene ID: 5071

UniProt: O60260

Pathways: Autophagy, Ubiquitin Proteasome Pathway