Patched 1 antibody (AA 1-80)

Catalog No. ABIN7223726

This Rabbit Polyclonal antibody specifically detects Patched 1 in WB, ELISA and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for Patched 1 antibody (AA 1-80) (ABIN7223726)

Target: Patched 1 (PTCH1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Patched 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Patched 1 Antibody

Binding Specificity: AA 1-80

Purpose: Patched Polyclonal Antibody

Specificity: Patched Polyclonal Antibody detects endogenous levels of Patched protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human Patched at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Patched 1

Target: Patched 1 (PTCH1)

Alternative Name: Patched

Background: Rabbit Anti-Patched Polyclonal Antibody,PTCH1, PTCH, Protein patched homolog 1, PTC, PTC1,PTCH1 (patched 1) encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of PTCH1 have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently.,Protein patched homolog 1

Molecular Weight: observerd band 160kDa

Gene ID: 5727

UniProt: Q13635

Pathways: Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation