ARHGEF11 antibody (AA 170-250)

Catalog No. ABIN7216492

This Rabbit Polyclonal antibody specifically detects ARHGEF11 in WB and IHC. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ARHGEF11 antibody (AA 170-250) (ABIN7216492)

Target: ARHGEF11 (rho Guanine Nucleotide Exchange Factor (GEF) 11 (ARHGEF11))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ARHGEF11 Antibody

Binding Specificity: AA 170-250

Purpose: Rabbit Anti-PDZ-RhoGEF Polyclonal Antibody

Specificity: PDZ-RhoGEF Polyclonal Antibody detects endogenous levels of PDZ-RhoGEF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human PDZ-RhoGEF at AA range: 170-250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARHGEF11

Target: ARHGEF11 (rho Guanine Nucleotide Exchange Factor (GEF) 11 (ARHGEF11))

Alternative Name: PDZ-RhoGEF

Background: ARHGEF11, KIAA0380, Rho guanine nucleotide exchange factor 11, PDZ-RhoGEFPeriphilin 1 encoded by PPHLN1 is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. Periphilin 1 interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of periphilin 1 suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

Gene ID: 9826

UniProt: O15085

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling