Pepsin A antibody (Internal Region)

Catalog No. ABIN7223130

The Rabbit Polyclonal anti-Pepsin A antibody (ABIN7223130) specifically detects Pepsin A in WB, IHC, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for Pepsin A antibody (Internal Region) (ABIN7223130)

Target: Pepsin A

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pepsin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-Pepsin A Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-Pepsin A Polyclonal Antibody

Specificity: Pepsin A Polyclonal Antibody detects endogenous levels of Pepsin A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Pepsin A

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Pepsin A

Target: Pepsin A

Background: Pepsin A, PGA3, PGA4, PGA5PGA5 (pepsinogen 5, group I (pepsinogen A)) encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. PGA5 is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer.

Molecular Weight: 42kD

Gene ID: 5222, 643847

UniProt: P00790