PPIA antibody (Internal Region)

Catalog No. ABIN7217932

This Rabbit Polyclonal antibody specifically detects PPIA in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for PPIA antibody (Internal Region) (ABIN7217932)

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PPIA Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CyPA Polyclonal Antibody

Specificity: CyPA Polyclonal Antibody detects endogenous levels of CyPA protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CyPA

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPIA

Target: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Alternative Name: CyPA

Background: PPIA, CYPA, Peptidyl-prolyl cis-trans isomerase A, PPIase A, Cyclophilin A, Cyclosporin A-binding protein, Rotamase APPIA encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The peptidylprolyl isomerase A is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.

Molecular Weight: 18kD

Gene ID: 5478

UniProt: P62937