PPIF antibody (AA 60-140)

Catalog No. ABIN7214408

The Rabbit Polyclonal anti-PPIF antibody (ABIN7214408) specifically detects PPIF in WB, IHC, ELISA and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PPIF antibody (AA 60-140) (ABIN7214408)

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PPIF Antibody

Binding Specificity: AA 60-140

Purpose: Rabbit Anti-Cyclophilin F Polyclonal Antibody

Specificity: Cyclophilin F Polyclonal Antibody detects endogenous levels of Cyclophilin F protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cyclophilin F at AA range: 60-140

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPIF

Target: PPIF (Peptidylprolyl Isomerase F (PPIF))

Alternative Name: Cyclophilin F

Background: PPIF, CYP3, Peptidyl-prolyl cis-trans isomerase F, mitochondrial, PPIase F, Cyclophilin F, Rotamase FPeptidylprolyl isomerase F encoded by PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

Molecular Weight: 22kD

Gene ID: 10105

UniProt: P30405

Pathways: Proton Transport, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity