PIGX antibody (AA 160-240)

Catalog No. ABIN7216560

The Rabbit Polyclonal anti-PIGX antibody is suitable to detect PIGX in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC and IF.

Quick Overview for PIGX antibody (AA 160-240) (ABIN7216560)

Target: PIGX (Phosphatidylinositol Glycan Anchor Biosynthesis, Class X (PIGX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIGX antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PIGX Antibody

Binding Specificity: AA 160-240

Purpose: Rabbit Anti-PIG-X Polyclonal Antibody

Specificity: PIG-X Polyclonal Antibody detects endogenous levels of PIG-X protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human PIG-X at AA range: 160-240

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PIGX

Target: PIGX (Phosphatidylinositol Glycan Anchor Biosynthesis, Class X (PIGX))

Alternative Name: PIG-X

Background: PIGX, Phosphatidylinositol-glycan biosynthesis class X protein, PIG-XPIGX encodes a type I transmembrane protein in the endoplasmic reticulum (ER). Phosphatidylinositol glycan anchor biosynthesis class X is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Alternative splicing results in multiple transcript variants.

Molecular Weight: 26kD

Gene ID: 54965

UniProt: Q8TBF5

Pathways: Inositol Metabolic Process