PFKP antibody (AA 310-390)

Catalog No. ABIN7216518

The Rabbit Polyclonal anti-PFKP antibody (ABIN7216518) specifically detects PFKP in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse, Rat and Monkey samples.

Quick Overview for PFKP antibody (AA 310-390) (ABIN7216518)

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PFKP Antibody

Binding Specificity: AA 310-390

Purpose: Rabbit Anti-PFK-C Polyclonal Antibody

Specificity: PFK-C Polyclonal Antibody detects endogenous levels of PFK-C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human PFK-C at AA range: 310-390

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PFKP

Target: PFKP (phosphofructokinase, Platelet (PFKP))

Alternative Name: PFK-C

Background: PFKP, PFKF, 6-phosphofructokinase type C, 6-phosphofructokinase, platelet type, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphofructokinase 1, PhosphohexokinaseThe PFKP gene encodes the platelet isoform of phosphofructokinase (PFK) (ATP: D-fructose-6-phosphate-1-phosphotransferase, EC 2. . 1). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. The PFKP gene, which maps to chromosome 10p, is also expressed in fibroblasts. See also the muscle (PFKM, MIM 610681) and liver (PFKL, MIM 171860) isoforms of phosphofructokinase, which map to chromosomes 12q13 and 21q22, respectively. Vora (1981) [PubMed 6451249] determined that full tetrameric phophofructokinase enzyme expressed in platelets can be composed of subunits P4, P3L, and P2L2.

Molecular Weight: 84kD

Gene ID: 5214

UniProt: Q01813

Pathways: Warburg Effect