PIK3R2 antibody (pTyr464)

Catalog No. ABIN7223152

The Rabbit Polyclonal anti-PIK3R2 antibody has been validated for WB and ELISA. It is suitable to detect PIK3R2 in samples from Human, Rat and Mouse.

Quick Overview for PIK3R2 antibody (pTyr464) (ABIN7223152)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PIK3R2 Antibody

Binding Specificity: pTyr464

Purpose: PI 3-kinase p85β (phospho Tyr464) Polyclonal Antibody

Specificity: Phospho-PI 3-kinase p85β (Y464) Polyclonal Antibody detects endogenous levels of PI 3-kinase p85β protein only when phosphorylated at Y464.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PI 3-kinase p85beta Phospho-Tyr464

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PI 3-kinase p85beta

Background: Rabbit Anti-PI 3-kinase p85β (phospho Tyr464) Polyclonal Antibody,PIK3R2, Phosphatidylinositol 3-kinase regulatory subunit beta, PI3-kinase regulatory subunit beta, PI3K regulatory subunit beta, PtdIns-3-kinase regulatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, PI3-kina,Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by PIK3CG (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma) is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p110-alpha p110-beta, and p110-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for PIK3CG.,Phosphatidylinositol 3-kinase regulatory subunit beta

Molecular Weight: observerd band 85kDa

Gene ID: 5296

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling