Phospholamban antibody

Catalog No. ABIN7223214

This anti-Phospholamban antibody is a Rabbit Polyclonal antibody detecting Phospholamban in ELISA and IF. Suitable for Human, Rat and Mouse.

Quick Overview for Phospholamban antibody (ABIN7223214)

Target: Phospholamban (PLN)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-Phospholamban Antibody

Purpose: PLB Polyclonal Antibody

Specificity: PLB Polyclonal Antibody detects endogenous levels of PLB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PLB around the non-phosphorylation site of S16/T17

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLB

Background: Rabbit Anti-PLB Polyclonal Antibody,PLN, PLB, Cardiac phospholamban, PLB,The protein encoded by PLN (phospholamban)is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in PLN are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.,Cardiac phospholamban

Gene ID: 5350

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity