Phospholamban antibody

Catalog No. ABIN7223214

The Rabbit Polyclonal anti-Phospholamban antibody (ABIN7223214) specifically detects Phospholamban in WB and IHC. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Phospholamban antibody (ABIN7223214)

Target: Phospholamban (PLN)

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Phospholamban Antibody

Purpose: Rabbit Anti-PLB Polyclonal Antibody

Specificity: PLB Polyclonal Antibody detects endogenous levels of PLB protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PLB around the non-phosphorylation site of S16/T17

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: PLB

Background: PLN, PLB, Cardiac phospholamban, PLBThe protein encoded by PLN (phospholamban)is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in PLN are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy.

Gene ID: 5350

UniProt: P26678

Pathways: Negative Regulation of Transporter Activity