PLCE1 antibody

Catalog No. ABIN7230680

This Rabbit Polyclonal antibody specifically detects PLCE1 in IHC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PLCE1 antibody (ABIN7230680)

Target: PLCE1 (Phospholipase C, epsilon 1 (PLCE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLCE1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PLCE1 Antibody

Purpose: Rabbit Anti-PLCE1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of PLCE1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human PLCE1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-300,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PLCE1

Target: PLCE1 (Phospholipase C, epsilon 1 (PLCE1))

Alternative Name: PLCE1

Background: 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase epsilon-1, Pancreas-enriched phospholipase C, Phosphoinositide phospholipase C-epsilon-1, Phospholipase C-epsilon-1, PLC-epsilon-1PLCE1 encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome, characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Molecular Weight: 253kD

Gene ID: 51196

UniProt: Q9P212

Pathways: EGFR Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling