PEA15 antibody

Catalog No. ABIN7225614

This Rabbit Polyclonal antibody specifically detects PEA15 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for PEA15 antibody (ABIN7225614)

Target: PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEA15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PEA15 Antibody

Purpose: Rabbit Anti-PEA-15 Polyclonal Antibody

Specificity: PEA-15 Polyclonal Antibody detects endogenous levels of PEA-15 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human PEA-15 around the non-phosphorylation site of S116

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PEA15

Target: PEA15 (phosphoprotein Enriched in Astrocytes 15 (PEA15))

Alternative Name: PEA-15

Background: PEA15, Astrocytic phosphoprotein PEA-15, 15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enriched in diabetes, PEDPEA15 (phosphoprotein enriched in astrocytes 15) encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants.

Molecular Weight: 36kD

Gene ID: 8682

UniProt: Q15121