STEAP3 antibody (AA 390-470)

Catalog No. ABIN7216538

The Rabbit Polyclonal anti-STEAP3 antibody is suitable to detect STEAP3 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for STEAP3 antibody (AA 390-470) (ABIN7216538)

Target: STEAP3 (STEAP Family Member 3, Metalloreductase (STEAP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STEAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-STEAP3 Antibody

Binding Specificity: AA 390-470

Purpose: Rabbit Anti-pHyde Polyclonal Antibody

Specificity:  pH yde Polyclonal Antibody detects endogenous levels of pH yde protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human pHyde at AA range: 390-470

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for STEAP3

Target: STEAP3 (STEAP Family Member 3, Metalloreductase (STEAP3))

Alternative Name: pHyde

Background: STEAP3, TSAP6, Metalloreductase STEAP3, Dudulin-2, Six-transmembrane epithelial antigen of prostate 3, Tumor suppressor-activated pathway protein 6, hTSAP6, pH yde, h pH ydePhosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in PIK3R1 has been associated with insulin resistance. Alternative splicing of PIK3R1 results in four transcript variants encoding different isoforms.

Molecular Weight: 54kD

Gene ID: 55240

UniProt: Q658P3

Pathways: Transition Metal Ion Homeostasis