PTAFR antibody (AA 170-250)

Catalog No. ABIN7223724

The Rabbit Polyclonal anti-PTAFR antibody is suitable to detect PTAFR in samples from Human, Mouse and Rat. It has been validated for ELISA and IF.

Quick Overview for PTAFR antibody (AA 170-250) (ABIN7223724)

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTAFR antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF)

Product Details anti-PTAFR Antibody

Binding Specificity: AA 170-250

Purpose: Rabbit Anti-PAF-R Polyclonal Antibody

Specificity: PAF-R Polyclonal Antibody detects endogenous levels of PAF-R protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human PAF-R at AA range: 170-250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications,

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PTAFR

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Alternative Name: PAF-R

Background: PTAFR, PAFR, Platelet-activating factor receptor, PAF-R, PAFrPTAFR (platelet activating factor receptor) encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants.

Gene ID: 5724

UniProt: P25105

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin