PARP1 antibody (Cleaved-Gly215)

Catalog No. ABIN7212502

This Rabbit Polyclonal antibody specifically detects PARP1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PARP1 antibody (Cleaved-Gly215) (ABIN7212502)

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PARP1 Antibody

Binding Specificity: AA 170-250, Cleaved-Gly215

Purpose: Cleaved-PARP-1 (G215) Polyclonal Antibody

Specificity: Cleaved-PARP-1 (G215) Polyclonal Antibody detects endogenous levels of fragment of activated PARP-1 protein resulting from cleavage adjacent to G215.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human PARP-1 at AA range: 170-250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PARP1

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Alternative Name: PARP-1

Background: Rabbit Anti-Cleaved-PARP-1 (G215) Polyclonal Antibody,PARP1, ADPRT, PPOL, Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1,PARP1 encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, poly(ADP-ribose) polymerase 1 may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.,Poly [ADP-ribose] polymerase 1

Molecular Weight: observerd band 89kDa

Gene ID: 142

UniProt: P09874

Pathways: Apoptosis, Caspase Cascade in Apoptosis, DNA Damage Repair, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location