POLR3C antibody (AA 160-240)

Catalog No. ABIN7219086

The Rabbit Polyclonal anti-POLR3C antibody is suitable to detect POLR3C in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for POLR3C antibody (AA 160-240) (ABIN7219086)

Target: POLR3C (Polymerase (RNA) III (DNA Directed) Polypeptide C (62kD) (POLR3C))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-POLR3C Antibody

Binding Specificity: AA 160-240

Purpose: Rabbit Anti-POLR3C Polyclonal Antibody

Specificity: POLR3C Polyclonal Antibody detects endogenous levels of POLR3C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human POLR3C at AA range: 160-240

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,IHC 1:50-300,ELISA 1:2000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLR3C

Target: POLR3C (Polymerase (RNA) III (DNA Directed) Polypeptide C (62kD) (POLR3C))

Alternative Name: POLR3C

Background: POLR3C, DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, DNA-directed RNA polymerase III subunit C, RNA polymerase III 62 kDa subunit, RPC62DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Molecular Weight: 60kD

Gene ID: 10623

UniProt: Q9BUI4