POLR3C antibody (Middle Region)

Catalog No. ABIN2784816

The Rabbit Polyclonal anti-POLR3C antibody is suitable to detect POLR3C in samples from Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit and Saccharomyces cerevisiae. It has been validated for WB.

Quick Overview for POLR3C antibody (Middle Region) (ABIN2784816)

Target: POLR3C (Polymerase (RNA) III (DNA Directed) Polypeptide C (62kD) (POLR3C))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3C antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-POLR3C Antibody

Binding Specificity: Middle Region

Purpose: POLR3C Antibody - middle region

Sequence: VEAIIASMQA TGAEEAQLQE IEEMITAPER QQLETLKRNV NKLDASEIQV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 90%

Characteristics: This is a rabbit polyclonal antibody against POLR3C. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human POLR3C

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52190-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR3C

Target: POLR3C (Polymerase (RNA) III (DNA Directed) Polypeptide C (62kD) (POLR3C))

Alternative Name: POLR3C

Background:

Targeted Subunit: RPC3

Background Information: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3C is a specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. POLR3C may direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. POLR3C may be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. POLR3C plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses.POLR3C acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3C can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway.

Gene Name: Polymerase (RNA) III (DNA directed) polypeptide C (62kD)

Alternative Symbols: C82, RPC3, RPC62

Protein Name: DNA-directed RNA polymerase III subunit RPC3

Molecular Weight: 60kDa

Gene ID: 10623

NCBI Accession: NP_006459

UniProt: Q9BUI4