POLR3F antibody

Catalog No. ABIN7231156

The Rabbit Polyclonal anti-POLR3F antibody is suitable to detect POLR3F in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for POLR3F antibody (ABIN7231156)

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3F antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLR3F Antibody

Purpose: Rabbit Anti-RPC6 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of RPC6 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human RPC6 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for POLR3F

Target: POLR3F (Polymerase (RNA) III (DNA Directed) Polypeptide F, 39 KDa (POLR3F))

Alternative Name: RPC6

Background: DNA-directed RNA polymerase III subunit RPC6, DNA-directed RNA polymerase III subunit F, RNA polymerase III 39 kDa subunitPOLR3F encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. POLR3F (RNA Polymerase III Subunit F) is a Protein Coding gene. Among its related pathways are Pyrimidine metabolism (KEGG) and RNA Polymerase III Transcription Initiation.

Molecular Weight: 34kD

Gene ID: 10621

UniProt: Q9H1D9