PNPT1 antibody (AA 540-620)

Catalog No. ABIN7216622

This Rabbit Polyclonal antibody specifically detects PNPT1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for PNPT1 antibody (AA 540-620) (ABIN7216622)

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNPT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PNPT1 Antibody

Binding Specificity: AA 540-620

Purpose: Rabbit Anti-PNPase Polyclonal Antibody

Specificity: PNPase Polyclonal Antibody detects endogenous levels of PNPase protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human PNPase at AA range: 540-620

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PNPT1

Target: PNPT1 (Polyribonucleotide Nucleotidyltransferase 1 (PNPT1))

Alternative Name: PNPase

Background: PNPT1, PNPASE, Polyribonucleotide nucleotidyltransferase 1, mitochondrial, 3'-5' RNA exonuclease OLD35, PNPase old-35, Polynucleotide phosphorylase 1, PNPase 1, Polynucleotide phosphorylase-like proteinPolyribonucleotide nucleotidyltransferase 1 encoded by PNPT1 elongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. Polyribonucleotide nucleotidyltransferase 1 is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in PNPT1 have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7.

Molecular Weight: 85kD

Gene ID: 87178

UniProt: Q8TCS8