PIAS3 antibody (AA 10-90)

Catalog No. ABIN7216554

This anti-PIAS3 antibody is a Rabbit Polyclonal antibody detecting PIAS3 in WB, ELISA and IHC (p). Suitable for Human, Rat and Mouse.

Quick Overview for PIAS3 antibody (AA 10-90) (ABIN7216554)

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIAS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIAS3 Antibody

Binding Specificity: AA 10-90

Purpose: PIAS 3 Polyclonal Antibody

Specificity: PIAS 3 Polyclonal Antibody detects endogenous levels of PIAS 3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human PIAS 3 at AA range: 10-90

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PIAS3

Target: PIAS3 (Protein Inhibitor of Activated STAT, 3 (PIAS3))

Alternative Name: PIAS 3

Background: Rabbit Anti-PIAS 3 Polyclonal Antibody,PIAS3, E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3,PIAS3 encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. Protein inhibitor of activated STAT 3 functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of PIAS3 have been identified, but the full-length nature of some of these variants has not been determined.,E3 SUMO-protein ligase PIAS3

Molecular Weight: observerd band 68kDa

Gene ID: 10401

UniProt: Q9Y6X2

Pathways: JAK-STAT Signaling