PKC antibody

Catalog No. ABIN7216584

This anti-PKC antibody is a Rabbit Polyclonal antibody detecting PKC in WB, ELISA, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for PKC antibody (ABIN7216584)

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PKC Antibody

Purpose: PKC Polyclonal Antibody

Specificity: PKC Polyclonal Antibody detects endogenous levels of PKC protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human PKC around the non-phosphorylation site of T497

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for PKC

Target: PKC (PKC pan) (Protein Kinase C (pan) (PKC pan))

Alternative Name: PKC

Background: Rabbit Anti-PKC Polyclonal Antibody,PRKCA, PKCA, PRKACA, Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCB, PKCB, PRKCB1, Protein kinase C beta type, PKC-B, PKC-beta, PRKCD, Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta, PRKCE, PKCE, Protein kinase,Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. Protein kinase C alpha by PRKCA is one of the PKC family members. Protein kinase C alphahas been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca (2+) handling in myocytes,Protein kinase C alpha type

Molecular Weight: observerd band 67-83kD(α 76,δ/β 77,γ 78, θ/ε83 , ζ 67)kDa

Gene ID: 5578, 5579, 5580

Pathways: Positive Regulation of Response to DNA Damage Stimulus