PKC eta antibody

Catalog No. ABIN7229318

The Rabbit Polyclonal anti-PKC eta antibody (ABIN7229318) specifically detects PKC eta in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PKC eta antibody (ABIN7229318)

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC eta antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PKC eta Antibody

Purpose: Rabbit Anti-KPCL Polyclonal Antibody

Specificity: The antibody detects endogenous levels of KPCL protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human KPCL protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PKC eta

Target: PKC eta (PRKCH) (Protein Kinase C, eta (PRKCH))

Alternative Name: KPCL

Background: Protein kinase C eta type, PKC-L, nPKC-etaProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. Protein Kinase C Eta encoded by PRKCH is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction.

Molecular Weight: 75kD

Gene ID: 5583

UniProt: P24723

Pathways: Myometrial Relaxation and Contraction, Thromboxane A2 Receptor Signaling